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Nitric oxide (NO) is a signalling molecule that tells your blood vessels to dilate. Wider vessels mean more oxygen and nutrients reach your muscles, waste products clear faster, and your perceived effort drops at any given pace. For Australian endurance athletes, runners chasing a Gold Coast Marathon PB, triathletes prepping for Ironman Cairns, cyclists putting in long road hours, that’s a measurable performance edge. Research shows beetroot juice supplementation can reduce the oxygen cost of submaximal exercise by around 3% in trained athletes.12
But here’s the part most blogs skip: nitric oxide doesn’t fuel your muscles. It improves delivery. If you don’t have steady energy in the system, all the vasodilation in the world won’t stop you emptying out at 32km. That’s why this guide covers both halves: how to raise NO, and how to keep your fuel steady so the gain actually pays off.
There are two real ways to raise nitric oxide. Most products on shelves use one of them. Some try to be both and end up doing neither well.
You eat dietary nitrates, concentrated beetroot juice or leafy greens. Bacteria on the back of your tongue convert nitrate to nitrite. Stomach acid converts nitrite to NO, which enters the bloodstream.3 Slow build up, sustained effect, best for long endurance events.
L-citrulline converts to L-arginine in your kidneys, which the enzyme eNOS turns directly into NO inside your blood vessels. Faster onset, stronger acute effect, best for high intensity sessions, intervals, criteriums and time trials.4
Why not just take L-arginine directly? Your gut and liver break it down before it can do its job. L-citrulline bypasses that and produces higher, more sustained arginine levels in the blood. This is settled science.
| Feature | Beetroot (Nitrate Pathway) | L-Citrulline (Arginine Pathway) |
|---|---|---|
| Source | Concentrated beetroot juice, leafy greens | L-citrulline malate powder |
| Mechanism | Mouth bacteria convert nitrate to nitrite to NO | Citrulline to arginine to NO via eNOS |
| Onset | 5 to 7 days of loading | 60 to 90 minutes acute |
| Best for | Marathon, long course tri, long ride | Track intervals, crits, 5 to 10 km races |
| Typical dose | 70 to 140 ml concentrated shot daily | 6 to 8 g, 60 to 90 min pre session |
The mistake I see constantly: athletes buy a beetroot shot the day before a race, drink it 2 hours before the start, and expect magic. That’s not how this works.
The protocol that’s backed by research:
This loading phase fits inside any standard race week plan. It doesn’t replace carb loading. It doesn’t replace your race morning fuelling. It runs alongside both.
This is where the post pivots, because beetroot is a delivery upgrade, not a fuel. On race morning you still need carbohydrate energy that’s going to last. Sugar based gels and drinks arrive fast and leave fast, usually 30 to 45 minutes later, which is exactly when most marathoners hit the wall.
UCAN Energy Powder and UCAN Energy + Protein use LIVSTEADY, a slow release carbohydrate originally developed as a medical food for children who could not reach the energy they had stored. It delivers energy at a steady rate across 75 minutes or more. Take it 30 to 45 minutes before your race, alongside your beetroot shot, and you’ve got both halves of the equation: dilated vessels carrying steady fuel.
Seven days of consistent endurance training plus a daily concentrated nitrate dose pulls hard on your fluid and electrolyte balance. UCAN Hydrate is a sugar free electrolyte drink purpose built for this: sodium, potassium, magnesium and calcium, zero added sugar, no jittery stimulants. A bottle on every long run during your loading phase keeps the rest of your system functioning while the nitrates do their work.
If beetroot is your endurance base layer, L-citrulline is your sharp end. It comes into its own when the session is short, hard, and demands repeat efforts: 5 to 10 km races, track repeats, criteriums, threshold blocks, hilly time trials.
The protocol:
A 2016 trial in trained cyclists showed 2.4 g/day of L-citrulline for 7 days improved 4 km time trial performance, and acute doses of 6 to 8 g around an hour pre session are well supported in the literature.45
Two different jobs, complementary biochemistry:
A sugar based gel with a high intensity NO product is a poor combination. You get the vasodilation, then the fuel is gone twenty minutes in and you’re back where you started partway through the session it was meant to sharpen. Slow release fuel holds across the whole block. That’s the whole point of the product.
Most pre workout products in Australia are stimulant delivery systems with other ingredients along for the ride. If you train in the evening, react badly to caffeine, or are already at your daily limit from coffee, that whole category is closed to you.
Beetroot and L-citrulline are the honest caffeine free pre workout. Neither is a stimulant, neither touches your sleep, and both have a mechanism you can point at and a dose you can measure.
Pair either with a serve of UCAN Energy Powder 30 to 45 minutes out and you have a complete stimulant free pre session routine: delivery from the nitric oxide side, fuel from the carbohydrate side, and nothing in it that will still be in your system at bedtime.
Remember pathway 1? It depends on bacteria on the back of your tongue converting nitrate to nitrite. Antiseptic mouthwash, anything with chlorhexidine or alcohol, kills those bacteria indiscriminately. You can spend $40 on a week of beetroot shots and wipe the entire effect out with one swish.6
Studies of the oral microbiome have repeatedly linked regular antiseptic mouthwash use to reduced nitric oxide production in athletes. The action is simple:
| Day / Time | Action |
|---|---|
| Day 7 to day 1 | Beetroot shot daily with breakfast |
| Race week | Drop antiseptic mouthwash, hydrate daily with UCAN Hydrate |
| Race morning, 3 hr out | Final beetroot shot plus breakfast |
| Race morning, 30 to 45 min out | UCAN Energy Powder or Energy + Protein |
| During the race | UCAN Edge gel every 45 to 60 min as needed |
| Time | Action |
|---|---|
| 90 min out | 6 to 8 g L-citrulline malate in water |
| 30 to 45 min out | UCAN Edge gel |
| Warm up | Sip UCAN Hydrate |
| Session | Hard efforts as planned |
Coach’s rule, no exceptions: Never trial a new supplement on race day. Test the full protocol, beetroot, the mouthwash swap and the fuel timing, during your hardest training week first.
We’re the official Australian reseller of UCAN. We don’t sell beetroot or L-citrulline. UCAN doesn’t make those products and we don’t on sell other brands. So if you came here looking for an Australian source of beetroot powder, this isn’t it. You’ll find good options at most supplement retailers.
What we do sell is the steady energy half of the equation. Most athletes treat fuel as an afterthought to the supplement they’re excited about, whether that’s NO products, beta alanine or creatine. That’s backwards. Your fuel is the foundation. Everything else stacks on top.
Theoretically, yes. Practically, you’d need 300 to 600 mg of nitrates daily, which is a lot of beetroot, rocket or spinach, and the volume causes most athletes gut problems. Concentrated shots exist for a reason.
Beeturia, meaning pink urine and stools, is the most common and it’s harmless. L-citrulline above 8 to 10 g may cause minor stomach upset in some people. Trial in training, never on race day.
Yes. They use different biological pathways and don’t interfere with each other. A common A race protocol: load beetroot for the week, take L-citrulline 60 minutes before the gun for an extra lift.
L-citrulline acts in 60 to 90 minutes. Beetroot is a 5 to 7 day build up, so you won’t feel a single dose meaningfully.
Beetroot and L-citrulline are the two with real evidence behind them, and neither is a stimulant. Pair either with a serve of UCAN Energy Powder 30 to 45 minutes before the session and you have a complete stimulant free routine that won’t still be in your system at bedtime.
No. UCAN is a slow release carbohydrate fuel based on LIVSTEADY. It’s designed to deliver energy at a steady rate rather than all at once, and it works alongside NO supplements rather than instead of them.
L-citrulline can help. Beetroot loading is overkill for anything under 60 minutes, since the loading effort isn’t worth the marginal gain.
Beetroot and L-citrulline improve the delivery system. Your engine still needs fuel that holds through the session. That’s where UCAN comes in.
At Generation UCAN Australia, we help Australian endurance athletes, runners, triathletes, cyclists and walk-runners, fuel and recover smarter. For more, see Edge Energy Gels, UCAN Hydrate, Energy Powder, and our full UCAN product range.
Guidance here is general and not individual advice. Individual response to nitrate and to L-citrulline varies widely. Test any change to your supplement or fuelling routine in training before you race it.
Four guides on what goes in alongside the supplement.
The slow release carbohydrate under the whole range, explained plainly.
Read the explainer Pre workoutWhat to eat 30, 60 and 120 minutes out, and what to leave alone.
Read the ideas CaffeineThe arithmetic on dose, and where a gel fits alongside your coffee.
Read the guide HydrationFour product types compared, and what to check on a label in thirty seconds.
Read the guide
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